Recombinant:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Blocking Assay:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Clinical Proteomics:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Electron Microscopy:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Antibody Labeling:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Staining:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Cell Isolation:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
RNA sequencing:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
Gene Expression:Article Title: Multiomic analysis of ART-interruption cohorts identifies cell-extrinsic and -intrinsic mechanisms driving lymphocyte-mediated control of HIV rebound.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples PBMCs from people with HIV ACTG A5345, REDUC, TEACH, CLEAR, N/A Tonsils from people without HIV Cooperative Human Tissue Network (CHTN) N/A PBMCs from people without HIV https://www.vitalant.org N/A Chemicals, peptides, and recombinant proteins Human TruStain FcXTM Fc Block BioLegend Cat# 422302; RRID: AB_2818986 Fetal Bovine Serum VWR Cat# 97068-085 RPMI-1640 medium Corning Cat# 10-040-CM Lymphoprep density gradient medium StemCell Technologies Cat# 07851 Penicillin-Streptomycin-Glutamine Thermo Fisher Scientific Cat# 10378016 Metal Contaminant-Free PBS Rockland Cat# MB-008 Normal Mouse Serum Thermo Fisher Scientific Cat# 10410 Normal Rat Serum Thermo Fisher Scientific Cat# 10710C Human Serum from Male AB Plasma Sigma-Aldrich Cat# H4522 Iridium Interchelator Solution Standard Biotools Cat# 201192B EQ Four Element Calibration Beads Standard Biotools Cat# 201078 BD CompBeads BD Biosciences Cat# 555843 16% Paraformaldehyde Electron Microscopy Sciences Cat# 15710 Antibiotic-Antimycotic Solution, 100X Corning Cat# 30-004-CI DNase I Sigma Cat# 11284932001 Phorbol 12-myristate 13-acetate (PMA) Selleckchemor Cat# S7791 Torin Selleckchem Cat# S2827 SMAP-2 MedChemExpress HY Cat# 120272 Metformin Sigma-Aldrich Cat# PHR1084 Puromycin Thermo Fisher Cat# A1113803 Doxycycline StemCell Technologies Cat# 72742 Critical commercial assays MaxPar X8 Antibody Labeling Kit Standard Biotools Cat# 201169B Cell-ID 20-Plex Pd Barcoding Kit Standard Biotools Cat# 205060 Foxp3 Transcription Factor Staining Buffer Set eBioscience Cat# 00-5523-00 MaxPar Cell Acquisition Solution Standard Biotools Cat# 201240 MaxPar PBS Standard Biotools Cat# 201058 MaxPar Cell Staining Buffer Standard Biotools Cat# 201068 LentiX p24Gag Rapid Titer Kit Takara Bio Inc Cat# 632200 Zombie AquaTM Fixable Viability Kit BioLegend Cat# 423102; NULISAseq Inflammation Panel 250 Alamar Biosciences https://alamarbio.com/products-and- services/nulisa-inflammation-panel/ EasySepTM Human CD4 + T Cell Isolation Kit StemCell Technologies Cat# 100-0696 NEB Next Ultra II RNA Library Prep Kit for Illumina New England Biolabs Cat# E7770 Deposited data Raw CyTOF datasets This paper http://datadryad.org/share/GGND55gKi_ JGqv-48c2uKc3yxMwKI7rioHbwm9FKRzY Raw RNAseq data This paper Gene Expression Omnibus (GEO) database under accession number GSE290636 Recombinant DNA pHIV-F4-HSA (pNL-HSA.6ATRiC109FPB4.ecto) Cavrois et al. (PMID 28746881) N/A (Continued on next page) e3 Immunity 59, 1–19.e1–e9, May 12, 2026 ..
other:
Concentration Assay:Article Title: Distinct transcriptional and epigenomic programs define Hofbauer cells in term placenta
Article Snippet: Samples were washed 2 times with MCSB and once with PBS, and then filtered through a 30 μm Celltrics gravity filter (Sysmex). .. The cell concentration was adjusted to 7 × 10 5 /mL in Cell Acquisition Solution (Standard BioTools) including EQ 4-element calibration beads (Standard BioTools) at a 1:10 ratio [v/v]. .. Applying the WB injector (Standard BioTools), cells were acquired using a properly tuned Helios mass cytometer (Standard BioTools).
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